Immunohistochemistry:Article Title: DNases improve effectiveness of antibiotic treatment in murine polymicrobial sepsis
Article Snippet: Supplement 1: Differences in immunohistochemistry. (HE-staining/Ly6G-staining) Supplement 2: Western blot analysis of various inflammation associated proteins in liver and lung tissue Western Blot: Primary Antibodies Western Blot: Secondary Antibodies Secondary Antibody Producer Dilution Goat-anti-Rabbit HRP Antibodies online (Aachen, Germany) ABIN3020597 1:10000 Goat-anti-Mouse HRP Antibodies online (Aachen, Germany) ABIN3020588 1:10000 Antibody Producer Dilution Tumor Necrosis Factor α Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #PA5-19810 1:500 Interleukin 1ß Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #MM425B 1:3000 Interleukin 10 Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #MM010 1:1000 Nuclear-Factor kB-p65 Abcam (Cambridge, UK) #ab16502 1:5000 CXCR1 Proteintech (Rosemont, Illinois, USA) #55450-1-AP 1:1000 GAPDH (Loading control) Abcam (Cambridge, UK) #ab181602 1:20000 Cyclophilin A (Loading control) Cell Signaling (Danvers, Massachusetts, USA) #2175S 1:10000 Antibody Producer Dilution Neutrophil Elastase (NE) Rabbit polyclonal Abcam (Cambridge, UK) #ab68672 1:100 NE iso control Rabbit polyclonal Abcam (Cambridge, UK) #ab37415 1:1000 Histone H3cit (R8-Clone) Rabbit monoclonal Abcam (Cambridge, UK) #ab219406 1:100 H3-cit iso control Rabbit monoclonal Abcam (Cambridge, UK) #ab172730 1:300 Myeloperoxidase (MPO) Goat polyclonal R&D Systems (Minneapolis, Minnesota, USA) #AF3667 1:20 MPO iso control Goat polyclonal R&D Systems (Minneapolis, Minnesota, USA) #AB-108-C 1:100 IF (I): Secondary Antibodies Antibody Producer Color Dilution NE donkey anti rabbit Jackson Immunoresearch Philadelphia, Pennsylvania, USA #711-605-152 AF 647 1:200 MPO donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #705-165-147 Cy3 1:200 H3cit donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #711-605-152 AF 647 1:200 IF (II): Primary Antibodies IF (II): Secondary Antibodies Antibody Producer Dilution Histone H3cit (R2, 8, 17-Clone) Rabbit polyclonal Abcam (Cambridge, UK) #ab281584 1:300 Myeloperoxidase (MPO) Rabbit polyclonal Abcam (Cambridge, UK) #ab9535 1:200 Neutrophil Elastase (NE) Goat polyclonal Santa Cruz (Dallas, Texas, USA) #Sc-9521 1:100 Antibody Producer Color Dilution IgG goat anti rabbit Jackson Immunoresearch Philadelphia, Pennsylvania, USA #111-175-144 Cy5 1:400 IgG goat anti rabbit Abcam (Cambridge, UK) #ab175471 AF 568 1:400 IgG donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #705-175-147 Cy5 1:400
Western Blot:Article Title: DNases improve effectiveness of antibiotic treatment in murine polymicrobial sepsis
Article Snippet: Supplement 1: Differences in immunohistochemistry. (HE-staining/Ly6G-staining) Supplement 2: Western blot analysis of various inflammation associated proteins in liver and lung tissue Western Blot: Primary Antibodies Western Blot: Secondary Antibodies Secondary Antibody Producer Dilution Goat-anti-Rabbit HRP Antibodies online (Aachen, Germany) ABIN3020597 1:10000 Goat-anti-Mouse HRP Antibodies online (Aachen, Germany) ABIN3020588 1:10000 Antibody Producer Dilution Tumor Necrosis Factor α Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #PA5-19810 1:500 Interleukin 1ß Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #MM425B 1:3000 Interleukin 10 Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #MM010 1:1000 Nuclear-Factor kB-p65 Abcam (Cambridge, UK) #ab16502 1:5000 CXCR1 Proteintech (Rosemont, Illinois, USA) #55450-1-AP 1:1000 GAPDH (Loading control) Abcam (Cambridge, UK) #ab181602 1:20000 Cyclophilin A (Loading control) Cell Signaling (Danvers, Massachusetts, USA) #2175S 1:10000 Antibody Producer Dilution Neutrophil Elastase (NE) Rabbit polyclonal Abcam (Cambridge, UK) #ab68672 1:100 NE iso control Rabbit polyclonal Abcam (Cambridge, UK) #ab37415 1:1000 Histone H3cit (R8-Clone) Rabbit monoclonal Abcam (Cambridge, UK) #ab219406 1:100 H3-cit iso control Rabbit monoclonal Abcam (Cambridge, UK) #ab172730 1:300 Myeloperoxidase (MPO) Goat polyclonal R&D Systems (Minneapolis, Minnesota, USA) #AF3667 1:20 MPO iso control Goat polyclonal R&D Systems (Minneapolis, Minnesota, USA) #AB-108-C 1:100 IF (I): Secondary Antibodies Antibody Producer Color Dilution NE donkey anti rabbit Jackson Immunoresearch Philadelphia, Pennsylvania, USA #711-605-152 AF 647 1:200 MPO donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #705-165-147 Cy3 1:200 H3cit donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #711-605-152 AF 647 1:200 IF (II): Primary Antibodies IF (II): Secondary Antibodies Antibody Producer Dilution Histone H3cit (R2, 8, 17-Clone) Rabbit polyclonal Abcam (Cambridge, UK) #ab281584 1:300 Myeloperoxidase (MPO) Rabbit polyclonal Abcam (Cambridge, UK) #ab9535 1:200 Neutrophil Elastase (NE) Goat polyclonal Santa Cruz (Dallas, Texas, USA) #Sc-9521 1:100 Antibody Producer Color Dilution IgG goat anti rabbit Jackson Immunoresearch Philadelphia, Pennsylvania, USA #111-175-144 Cy5 1:400 IgG goat anti rabbit Abcam (Cambridge, UK) #ab175471 AF 568 1:400 IgG donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #705-175-147 Cy5 1:400
Control:Article Title: DNases improve effectiveness of antibiotic treatment in murine polymicrobial sepsis
Article Snippet: Supplement 1: Differences in immunohistochemistry. (HE-staining/Ly6G-staining) Supplement 2: Western blot analysis of various inflammation associated proteins in liver and lung tissue Western Blot: Primary Antibodies Western Blot: Secondary Antibodies Secondary Antibody Producer Dilution Goat-anti-Rabbit HRP Antibodies online (Aachen, Germany) ABIN3020597 1:10000 Goat-anti-Mouse HRP Antibodies online (Aachen, Germany) ABIN3020588 1:10000 Antibody Producer Dilution Tumor Necrosis Factor α Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #PA5-19810 1:500 Interleukin 1ß Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #MM425B 1:3000 Interleukin 10 Thermo-Fisher Scientific (Waltham, Massachussetts, USA) #MM010 1:1000 Nuclear-Factor kB-p65 Abcam (Cambridge, UK) #ab16502 1:5000 CXCR1 Proteintech (Rosemont, Illinois, USA) #55450-1-AP 1:1000 GAPDH (Loading control) Abcam (Cambridge, UK) #ab181602 1:20000 Cyclophilin A (Loading control) Cell Signaling (Danvers, Massachusetts, USA) #2175S 1:10000 Antibody Producer Dilution Neutrophil Elastase (NE) Rabbit polyclonal Abcam (Cambridge, UK) #ab68672 1:100 NE iso control Rabbit polyclonal Abcam (Cambridge, UK) #ab37415 1:1000 Histone H3cit (R8-Clone) Rabbit monoclonal Abcam (Cambridge, UK) #ab219406 1:100 H3-cit iso control Rabbit monoclonal Abcam (Cambridge, UK) #ab172730 1:300 Myeloperoxidase (MPO) Goat polyclonal R&D Systems (Minneapolis, Minnesota, USA) #AF3667 1:20 MPO iso control Goat polyclonal R&D Systems (Minneapolis, Minnesota, USA) #AB-108-C 1:100 IF (I): Secondary Antibodies Antibody Producer Color Dilution NE donkey anti rabbit Jackson Immunoresearch Philadelphia, Pennsylvania, USA #711-605-152 AF 647 1:200 MPO donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #705-165-147 Cy3 1:200 H3cit donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #711-605-152 AF 647 1:200 IF (II): Primary Antibodies IF (II): Secondary Antibodies Antibody Producer Dilution Histone H3cit (R2, 8, 17-Clone) Rabbit polyclonal Abcam (Cambridge, UK) #ab281584 1:300 Myeloperoxidase (MPO) Rabbit polyclonal Abcam (Cambridge, UK) #ab9535 1:200 Neutrophil Elastase (NE) Goat polyclonal Santa Cruz (Dallas, Texas, USA) #Sc-9521 1:100 Antibody Producer Color Dilution IgG goat anti rabbit Jackson Immunoresearch Philadelphia, Pennsylvania, USA #111-175-144 Cy5 1:400 IgG goat anti rabbit Abcam (Cambridge, UK) #ab175471 AF 568 1:400 IgG donkey anti goat Jackson Immunoresearch Philadelphia, Pennsylvania, USA #705-175-147 Cy5 1:400
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